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antibody against p-pi3k (tyr607  (Thermo Fisher)


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    Structured Review

    Thermo Fisher antibody against p-pi3k (tyr607
    Antibody Against P Pi3k (Tyr607, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p%E2%80%90pi3k+(tyr607/pm37606338-39-40-45
    Average 90 stars, based on 1 article reviews
    antibody against p-pi3k (tyr607 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Staining:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Immunostaining:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Over Expression:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Migration:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    In Vitro:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Western Blot:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Transwell Assay:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    In Vivo:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Inhibition:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Injection:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Microscopy:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Expressing:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Activation Assay:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Incubation:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Saline:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Control:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.

    Blocking Assay:

    Article Title: Vildagliptin attenuates acetic acid-induced colitis in rats via targeting PI3K/Akt/NFκB, Nrf2 and CREB signaling pathways and the expression of lncRNA IFNG-AS1 and miR-146a.
    Article Snippet: Inflammatory processes, including ulcerative colitis (UC), are associated with the increase in synthesis and release of pro-inflammatory cytokines.. The release of these cytokines is regulated by phosphatidylinositol-3kinase (PI3K)/protein kinase B (Akt)/nuclear factor-kappa B (NFκB) and cAMP response element-binding protein (CREB) signaling pathways as well as over expression of microRNA 146a (miR-146a) and long non-coding RNA interferon gamma antisense 1 (lncRNA IFNG-AS1).. Vildagliptin (Vilda), a dipeptidyl peptidase IV (DPP-IV) inhibitor, has an anti-inflammatory, antioxidant and anti-apoptotic effects which were established in various models.

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP‐C (1:200 dilution, Abcam, ab90716), Ki‐67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:200 dilution, CST, #4691), p‐AKT (Ser473; 1:100 dilution, CST, #4060), mTOR (1:100 dilution, CST, #2983), p‐mTOR (Ser2448; 1:200 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3 (1:200 dilution, Huabio, ET1602‐39), Cleaved‐Caspase‐3 (1:200 dilution, Affinity, AF7002), Bcl‐2 (1:200 dilution, Huabio, ET1603‐11), and Bax (1:200 dilution, Affinity, AF0120).

    Article Title: Selenium-binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non-small cell lung cancer.
    Article Snippet: In IHC staining, the sections were deparaffinize, rehydrated, followed by antigen retrieval and immunostaining with different antibodies against SELENBP1 (1:200 dilution, Abcam, ab90135), SP- C (1:200 dilution, Abcam, ab90716), Ki- 67 (1:200 dilution, Abcam, ab15580), PI3K (1:100 dilution, Abcam, ab151549), p- PI3K (Tyr607; 1:200 dilution, Invitrogen, Catalog #PA5- 104853), AKT 20457634, 2023, 16, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1002/cam 4.6309 by Indian Institute O f T echnology, K haragpur C entral L ibrary, W iley O nline L ibrary on [20/01/2024].

    Article Title: Protective effect of alogliptin against cyclophosphamide-induced lung toxicity in rats: Impact on PI3K/Akt/FoxO1 pathway and downstream inflammatory cascades
    Article Snippet: Afterwards, we incubate these membranes with polyclonal antibodies (1:1000): p-Akt (Thr450) was obtained from [Thermo Fisher Scientific, catalog number # PA5-37,469, RRID AB_2554078], p-PI3K (Tyr607) from [Thermo Fisher Scientific, catalog number # PA5-38,905, RRID AB_2555497] and p-FoxO1 (Ser249) attained by [Thermo Fisher Scientific, catalog number # PA5-64,676, RRID AB_2661988].

    Article Title: Selenium‐binding protein 1 inhibits malignant progression and induces apoptosis via distinct mechanisms in non‐small cell lung cancer
    Article Snippet: Primary antibodies against β‐actin (1:1000 dilution, Abcam, ab8226), SELENBP1 (1:1000 dilution, Abcam, ab90135), PI3K (1:1000 dilution, Abcam, ab151549), p‐PI3K (Tyr607; 1:1000 dilution, Invitrogen, Catalog #PA5‐104853), AKT (1:1000 dilution, CST, #4691), p‐AKT (Ser473; 1:2000 dilution, CST, #4060), mTOR (1:1000 dilution, CST, #2983), p‐mTOR (Ser2448; 1:1000 dilution, Invitrogen, Catalog #44‐1125G), Caspase‐3/p17 (1:1000 dilution, Proteintech, 66,470‐2‐Ig), Bcl‐2 (1:1000 dilution, Huabio, ET1603‐11), and Bax (1:1000 dilution, Affinity, AF0120) were used.



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    Thermo Fisher antibody against p-pi3k (tyr607
    NCAPD3 upregulated the phosphorylation level of AKT (T308) via <t>JAK2/PI3K</t> axis. (A) Protein levels of PI3K p110β, PI3K p85, AKT, PTEN, p‐PI3K p85, and p‐AKT (T308) assayed by Western blot in NCAPD3 overexpression or knockdown PCa cells. (B) Protein expressions of NCAPD3, AKT, p‐AKT (T308), FOXO1, and p‐FOXO1 (S256) in DU145 cells with transfection of NCAPD3 and treatment of LY294002 (20 μM). (C and D) Levels of JAK2 protein and mRNA expressions in NCAPD3‐overexpression DU145 cells and NCAPD3‐knockdown LNCaP cells. (E) Protein levels of NCAPD3, PI3K, p‐PI3K, AKT, and p‐AKT (T308) in DU145 cells with transfection of NCAPD3 and treatment of Z3 (30 μM). Values are means ± SE from n = 3 independent repetitions, * p < 0.05, ** p < 0.01, *** p < 0.001, based on Student's t ‐test.
    Antibody Against P Pi3k (Tyr607, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p%E2%80%90pi3k+(tyr607/pm37606338-39-40-45
    Average 90 stars, based on 1 article reviews
    antibody against p-pi3k (tyr607 - by Bioz Stars, 2026-09
    90/100 stars
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    PCR primers and conditions

    Journal: Biomolecules and Biomedicine

    Article Title: Therapeutic potential of simvastatin in ALS: Enhanced axonal integrity and motor neuron survival through Apoa4 and Alb modulation

    doi: 10.17305/bb.2024.11218

    Figure Lengend Snippet: PCR primers and conditions

    Article Snippet: Primary antibodies: C4a (1:3000, 22233-1-AP, Proteintech, Wuhan, China); C5 (1:600, A8104, Abclonal, Wuhan, China); C3 (1:10,000, 21337-1-AP, Proteintech); PI3K (1:1000, ab302958, Abcam, UK); p-PI3K p85 alpha (Tyr607) (1:1000, AF3241, Affinity, Jiangsu, China); Alb (1:10,000, 16475-1-AP, Proteintech); p-AKT (Ser473) (1:10,000, 66444-1-Ig, Proteintech); AKT (1:5000, 60203-2-Ig, Proteintech); PPARγ (1:5000, 16643-1-AP, Proteintech); APOA4 (1:8000, 17996-1-AP, Proteintech); C1qB (1:1000, A5339, Abclonal, Wuhan, China); CRP (1:2000, 66250-1-Ig, Proteintech); and GAPDH (1:10,000, 60004-1-Ig, Proteintech).

    Techniques:

    Expression of mRNA and proteins. qRT-PCR results presented the expression levels of PI3K (A), AKT (B), C5 (C), PPARγ (D), C4a (E), C3 (F), C1qB (G), and CRP (H) mRNA in different groups; the expression levels of PI3K, p-PI3K, p-AKT, AKT, PPARγ, Apoa4 (I), and C4a, C5, C3, Alb, C1qB, and CRP (J) in different groups were detected. The levels of p-PI3K, p-AKT, PPARγ, Apoa4, C5, and Alb decreased, while C4a, C3, C1qB, and CRP increased in the SOD1G93A + PBS group compared with the WT group; * P < 0.05, ** P < 0.01, vs WT group; # P < 0.05, ## P < 0.01, vs SOD1 G93A + PBS group. qRT-PCR: Quantitative real-time polymerase chain reaction; SOD1: Superoxide dismutase 1; WT: Wilt-type; PPAR: Peroxisome proliferator-activated receptor.

    Journal: Biomolecules and Biomedicine

    Article Title: Therapeutic potential of simvastatin in ALS: Enhanced axonal integrity and motor neuron survival through Apoa4 and Alb modulation

    doi: 10.17305/bb.2024.11218

    Figure Lengend Snippet: Expression of mRNA and proteins. qRT-PCR results presented the expression levels of PI3K (A), AKT (B), C5 (C), PPARγ (D), C4a (E), C3 (F), C1qB (G), and CRP (H) mRNA in different groups; the expression levels of PI3K, p-PI3K, p-AKT, AKT, PPARγ, Apoa4 (I), and C4a, C5, C3, Alb, C1qB, and CRP (J) in different groups were detected. The levels of p-PI3K, p-AKT, PPARγ, Apoa4, C5, and Alb decreased, while C4a, C3, C1qB, and CRP increased in the SOD1G93A + PBS group compared with the WT group; * P < 0.05, ** P < 0.01, vs WT group; # P < 0.05, ## P < 0.01, vs SOD1 G93A + PBS group. qRT-PCR: Quantitative real-time polymerase chain reaction; SOD1: Superoxide dismutase 1; WT: Wilt-type; PPAR: Peroxisome proliferator-activated receptor.

    Article Snippet: Primary antibodies: C4a (1:3000, 22233-1-AP, Proteintech, Wuhan, China); C5 (1:600, A8104, Abclonal, Wuhan, China); C3 (1:10,000, 21337-1-AP, Proteintech); PI3K (1:1000, ab302958, Abcam, UK); p-PI3K p85 alpha (Tyr607) (1:1000, AF3241, Affinity, Jiangsu, China); Alb (1:10,000, 16475-1-AP, Proteintech); p-AKT (Ser473) (1:10,000, 66444-1-Ig, Proteintech); AKT (1:5000, 60203-2-Ig, Proteintech); PPARγ (1:5000, 16643-1-AP, Proteintech); APOA4 (1:8000, 17996-1-AP, Proteintech); C1qB (1:1000, A5339, Abclonal, Wuhan, China); CRP (1:2000, 66250-1-Ig, Proteintech); and GAPDH (1:10,000, 60004-1-Ig, Proteintech).

    Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

    NCAPD3 upregulated the phosphorylation level of AKT (T308) via JAK2/PI3K axis. (A) Protein levels of PI3K p110β, PI3K p85, AKT, PTEN, p‐PI3K p85, and p‐AKT (T308) assayed by Western blot in NCAPD3 overexpression or knockdown PCa cells. (B) Protein expressions of NCAPD3, AKT, p‐AKT (T308), FOXO1, and p‐FOXO1 (S256) in DU145 cells with transfection of NCAPD3 and treatment of LY294002 (20 μM). (C and D) Levels of JAK2 protein and mRNA expressions in NCAPD3‐overexpression DU145 cells and NCAPD3‐knockdown LNCaP cells. (E) Protein levels of NCAPD3, PI3K, p‐PI3K, AKT, and p‐AKT (T308) in DU145 cells with transfection of NCAPD3 and treatment of Z3 (30 μM). Values are means ± SE from n = 3 independent repetitions, * p < 0.05, ** p < 0.01, *** p < 0.001, based on Student's t ‐test.

    Journal: FASEB BioAdvances

    Article Title: NCAPD3 ‐mediated AKT activation regulates prostate cancer progression

    doi: 10.1096/fba.2024-00073

    Figure Lengend Snippet: NCAPD3 upregulated the phosphorylation level of AKT (T308) via JAK2/PI3K axis. (A) Protein levels of PI3K p110β, PI3K p85, AKT, PTEN, p‐PI3K p85, and p‐AKT (T308) assayed by Western blot in NCAPD3 overexpression or knockdown PCa cells. (B) Protein expressions of NCAPD3, AKT, p‐AKT (T308), FOXO1, and p‐FOXO1 (S256) in DU145 cells with transfection of NCAPD3 and treatment of LY294002 (20 μM). (C and D) Levels of JAK2 protein and mRNA expressions in NCAPD3‐overexpression DU145 cells and NCAPD3‐knockdown LNCaP cells. (E) Protein levels of NCAPD3, PI3K, p‐PI3K, AKT, and p‐AKT (T308) in DU145 cells with transfection of NCAPD3 and treatment of Z3 (30 μM). Values are means ± SE from n = 3 independent repetitions, * p < 0.05, ** p < 0.01, *** p < 0.001, based on Student's t ‐test.

    Article Snippet: Antibodies against p‐FoxO1(Ser256) (Cat#AF3417), RICTOR (Cat#DF7530), RAPTOR (Cat#DF7527), PI3K p85α (Cat#AF6241), p‐PI3K p85α (Tyr607) (Cat#AF3241), and p‐STAT3(Y705) (Cat#AF3293) were from Affinity Biosciences (Cincinnati, OH, USA).

    Techniques: Western Blot, Over Expression, Knockdown, Transfection

    NCAPD3 increased the level of STAT3 and recruited more STAT3 to the promoters of EZH2 and JAK2 and then activated EZH2/NSD2/mTORC2 and JAK2/PI3K pathways, which phosphorylated AKT at Thr 308 and Ser 473. Moreover, there is a positive mutual activation between STAT3 and JAK2, further enhanced by NCAPD3 to promote PCa progression. The graph was drawn by Figdraw.

    Journal: FASEB BioAdvances

    Article Title: NCAPD3 ‐mediated AKT activation regulates prostate cancer progression

    doi: 10.1096/fba.2024-00073

    Figure Lengend Snippet: NCAPD3 increased the level of STAT3 and recruited more STAT3 to the promoters of EZH2 and JAK2 and then activated EZH2/NSD2/mTORC2 and JAK2/PI3K pathways, which phosphorylated AKT at Thr 308 and Ser 473. Moreover, there is a positive mutual activation between STAT3 and JAK2, further enhanced by NCAPD3 to promote PCa progression. The graph was drawn by Figdraw.

    Article Snippet: Antibodies against p‐FoxO1(Ser256) (Cat#AF3417), RICTOR (Cat#DF7530), RAPTOR (Cat#DF7527), PI3K p85α (Cat#AF6241), p‐PI3K p85α (Tyr607) (Cat#AF3241), and p‐STAT3(Y705) (Cat#AF3293) were from Affinity Biosciences (Cincinnati, OH, USA).

    Techniques: Activation Assay